Biotechnology

Front

What is the purpose of restriction enzymes?

Back

To cut DNA at specific recognition sites

Front

What is the function of DNA ligase?

Back

To join fragments of DNA

Front

What is the function of a plasmid in DNA cloning?

Back

Serving as a vector to carry DNA into host cells

Front

What is the term for the resulting DNA molecule when two different DNA fragments are joined together in DNA cloning?

Back

Recombinant DNA

Front

What is the purpose of bacterial transformation in DNA cloning?

Back

To introduce foreign DNA into bacteria

Front

What is the goal of PCR?

Back

To amplify a specific DNA region

Front

What is denaturation in the PCR process?

Back

The separation of DNA strands by heating

Front

What is the typical temperature range for the denaturation step in PCR?

Back

90-95°C

Front

What is the purpose of the extension step in PCR?

Back

To synthesize new DNA strands

Front

How many cycles of denaturation, annealing, and extension are typically performed in a PCR reaction?

Back

20-40 cycles