Quick 10Q: BioTechnology

Front

How are a plasmid and gene of interest combined to create a vector?

Back

A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkage

Front

Why is Taq polymerase required for PCR?

Back

The process of PCR requires extreme high temperatures that would denature other polymerases.

Front

Orientation of DNA in gel electrophoresis?

Back

Large fragments are found near the well while small fragments are found at the other end.

Front

Why are some bands darker in a gel electrophoresis?

Back

There are a large amount of fragments the same size

Front

If one fragment was placed in the thermocycler for a PCR experiment, how many fragments would exist after 5 cycles?

Back

32

Front

Why must primers be added to the thermocycler during a PCR?

Back

Polymerase requires an open 3' end

Front

Function of -plasmid/LB and +plasmid/LB plates in a transformation experiment?

Back

To ensure the bacteria is viable after experimental set-up

Front

Minimum number of restriction sites on a plasmid if there are 3 fragments on the gel electrophoresis?

Back

3

Front

What determines the order of nucleotides in a DNA molecule?

Back

DNA sequencing

Front

Which of the following allow for amplification of a DNA fragment abiotically?
Options: DNA sequencing, Gel electrophoresis, Polymerase Chain Reaction, Bacterial Transformation

Back

Polymerase Chain Reaction