Module 6 Part 2

Front

What is the origin of replication in DNA replication?

Back

The origin of replication is the specific location on the DNA molecule where DNA replication begins, often referred to as 'oric'.

Front

What medium was used to grow the E. coli colony in the Meselson-Stahl experiment?

Back

The Meselson-Stahl experiment used a 15N-labeled medium to grow the E. coli colony.

Front

What type of bond forms between dTTP and dATP during DNA synthesis?

Back

A hydrogen bond forms between dTTP and dATP during DNA synthesis.

Front

What is the significance of the Meselson-Stahl experiment in molecular biology?

Back

The Meselson-Stahl experiment demonstrated the semi-conservative nature of DNA replication.

Front

Define semi-conservative replication.

Back

Semi-conservative replication is the process by which DNA is replicated in such a way that each new DNA molecule consists of one original strand and one newly synthesized strand.

Front

What is the role of helicase in DNA replication?

Back

Helicase is an enzyme that unwinds the DNA double helix at the replication fork, allowing the two strands to be copied.

Front

What are Okazaki fragments?

Back

Okazaki fragments are short sequences of DNA nucleotides synthesized discontinuously on the lagging strand during DNA replication.

Front

What is the function of DNA polymerase?

Back

DNA polymerase is the enzyme responsible for synthesizing new DNA strands by adding nucleotides complementary to the template strand.

Front

What is the difference between leading and lagging strands in DNA replication?

Back

The leading strand is synthesized continuously in the direction of the replication fork, while the lagging strand is synthesized discontinuously in short segments away from the fork.

Front

What is the role of primase in DNA replication?

Back

Primase is an enzyme that synthesizes a short RNA primer to provide a starting point for DNA polymerase to begin DNA synthesis.