Chapter 13

Front

What happens to a growing DNA chain after a ddNTP is added?

Back

The chain growth stops.

Front

What sequencing technique utilizes ddNTPs?

Back

Sanger sequencing.

Front

What is the name of J. Craig Venter’s sequencing center in Maryland?

Back

The Institute for Genomic Research (TIGR).

Front

What technique separates molecules by size using a gel under an electrical current?

Back

Gel electrophoresis.

Front

What type of nucleotides were traditionally used to visualize bands in DNA sequencing gels?

Back

Radioactive nucleotides.

Front

What is the resulting image called when using radioactive nucleotides to visualize DNA bands?

Back

Autoradiograph.

Front

What is the role of ddNTPs in DNA sequencing?

Back

They terminate the DNA chain elongation.

Front

What is the principle behind gel electrophoresis?

Back

Molecules are separated based on their size and charge.

Front

What is the significance of Sanger sequencing in genomics?

Back

It was the first method used for DNA sequencing and is still widely used for its accuracy.

Front

What are the components of a Sanger sequencing reaction?

Back

Template DNA, primers, DNA polymerase, dNTPs, and ddNTPs.