Immuno Lab Exam 1

Front

What is the primary purpose of the washing steps in an indirect EIA?

Back

To ensure that only specific binding is measured, thereby increasing the assay’s specificity.

Front

What happens if you forget to wash the wells after adding the conjugate in an indirect EIA?

Back

The color signal will be higher than usual.

Front

Why does skipping the wash step between conjugate and substrate increase color intensity?

Back

The unbound conjugate remains and reacts with the substrate.

Front

What is the consequence of omitting all washing steps in an indirect EIA?

Back

The assay would have high background noise due to unbound antibodies.

Front

How is agglutination inhibition result interpreted?

Back

Lack of agglutination is a positive test, indicating the presence of antigen in the patient sample.

Front

What is an indirect EIA?

Back

An enzyme immunoassay where the detection of the target antigen is achieved through a secondary antibody conjugated to an enzyme.

Front

What role does the conjugate play in an indirect EIA?

Back

The conjugate binds to the primary antibody and produces a measurable signal when the substrate is added.

Front

What is the significance of specificity in an indirect EIA?

Back

Specificity ensures that the assay accurately measures the target antigen without interference from other substances.

Front

What can cause high background noise in an indirect EIA?

Back

Unbound antibodies or conjugates that are not washed away can lead to high background noise.

Front

What is the function of the substrate in an indirect EIA?

Back

The substrate reacts with the enzyme linked to the conjugate to produce a detectable signal.