Chapter 13

Front

What is the name of Craig’s totally awesome yacht?

Back

sorcerer 2

Front

What instrument is used to shred DNA into 500-800 bp sections during shotgun sequencing?

Back

sonicator

Front

Why is the size of 500-800 bp chosen for DNA shredding in shotgun sequencing?

Back

It allows for efficient sequencing and assembly.

Front

What is the ratio of special nucleotides added during a sequencing reaction relative to normal dNTPs?

Back

10:1

Front

What does the Combined DNA Index System (CODIS) contain?

Back

DNA profiles belonging to many different individuals.

Front

What is the purpose of the Combined DNA Index System (CODIS)?

Back

To identify individuals with a very high probability.

Front

What special kind of nucleotide is used in the first generation of DNA sequencing?

Back

ddNTP (Dideoxynucleotide triphosphates).

Front

What does ddNTP stand for?

Back

Dideoxynucleotide triphosphates.

Front

What is the role of dideoxynucleotide triphosphates (ddNTPs) in DNA sequencing?

Back

They terminate DNA strand elongation during sequencing.

Front

What is shotgun sequencing?

Back

A method of sequencing DNA by randomly breaking it into small pieces and then assembling the sequences.