CRISPR Flashcard

Front

What does the acronym CRISPR stand for?

Back

Clustered Regularly-Interspaced Short Palindromic Repeats

Front

What organisms naturally have CRISPR?

Back

Bacteria

Front

What is the function of the Cas9 protein in CRISPR?

Back

Cut DNA

Front

What is the role of guide RNA in the CRISPR process?

Back

Ensures that the Cas9 enzyme cuts at the right point in the genome.

Front

How does guide RNA help the cell know where to cut the DNA?

Back

Guide RNA is complementary to the DNA sequence.

Front

What is the significance of CRISPR in genetic engineering?

Back

CRISPR allows for precise editing of DNA, enabling targeted modifications.

Front

What are the potential applications of CRISPR technology?

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Gene therapy, agriculture, and disease research.

Front

What is the difference between CRISPR and traditional genetic modification techniques?

Back

CRISPR is more precise and efficient than traditional methods.

Front

What are off-target effects in CRISPR?

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Unintended cuts in the DNA that can lead to mutations.

Front

What is the importance of the PAM sequence in CRISPR?

Back

The PAM sequence is required for Cas9 to bind and cut the DNA.