Biotechnology Ch. #8 MEGA REVIEW

Front

What is the role of a Quality Control (QC) department in biotechnology manufacturing?

Back

The QC department conducts tests and provides verification to ensure the quality and safety of the products being manufactured.

Front

Define transformation in the context of biotechnology.

Back

Transformation is the process by which a cell takes up and expresses foreign DNA.

Front

What is transduction in genetic engineering?

Back

Transduction is the process of using viruses to transform or genetically engineer cells.

Front

What wavelength of light is used by a UV spectrophotometer to measure DNA concentration?

Back

260 nm is the wavelength of light used to measure DNA concentration.

Front

What is a primer in the context of DNA replication?

Back

A primer is a short strand of DNA that is necessary to initiate DNA synthesis.

Front

Explain the importance of the QC department in biotechnology.

Back

The QC department ensures that products meet regulatory standards and are safe for use, which is critical in biotechnology.

Front

What are the key steps involved in the transformation process?

Back

The key steps include preparation of competent cells, introduction of foreign DNA, and selection of transformed cells.

Front

How do viruses facilitate transduction in genetic engineering?

Back

Viruses can carry foreign DNA into host cells, allowing for the genetic modification of those cells.

Front

What is the significance of measuring DNA concentration at 260 nm?

Back

Measuring DNA concentration at 260 nm is significant because nucleic acids absorb UV light at this wavelength, allowing for quantification.

Front

What role do primers play in PCR (Polymerase Chain Reaction)?

Back

Primers are essential in PCR as they provide a starting point for DNA polymerase to synthesize new DNA strands.