CCP Biology DNA TECHNOLOGY

Front

What is the name of the enzyme used in Polymerase Chain Reaction (PCR)?

Back

Taq polymerase

Front

What must be true for a human gene and a plasmid to be successfully combined?

Back

Both must be cut by the same restriction enzyme.

Front

At what temperature does the Extension step of PCR occur?

Back

72°C

Front

What is NOT a reason to genetically modify plants?

Back

To produce fewer plants.

Front

During gel electrophoresis, in which direction does DNA move?

Back

From the negative end to the positive end.

Front

What is the purpose of the Polymerase Chain Reaction (PCR)?

Back

To amplify specific DNA sequences.

Front

What is a restriction enzyme?

Back

An enzyme that cuts DNA at specific sequences.

Front

What is gel electrophoresis used for?

Back

To separate DNA fragments based on size.

Front

What is the role of primers in PCR?

Back

Primers are short sequences of nucleotides that initiate DNA synthesis.

Front

What is the significance of the 72°C temperature in PCR?

Back

It is the optimal temperature for Taq polymerase to extend the DNA strand.