Chromatograph_IB _LAB

Front

What is paper chromatography?

Back

A technique used to separate mixtures based on the different rates at which their components move through a stationary phase (the paper) and a mobile phase (the solvent).

Front

What is the stationary phase in paper chromatography?

Back

The paper itself.

Front

What is the mobile phase in paper chromatography?

Back

The solvent used to carry the components of the mixture.

Front

What factors can affect the separation in paper chromatography?

Back

Solvent, type of paper, temperature, size of molecules.

Front

What does the Rf value represent in chromatography?

Back

The ratio of the distance traveled by a component to the distance traveled by the solvent front.

Front

How is the Rf value calculated?

Back

Rf = (Distance traveled by the substance) / (Distance traveled by the solvent front).

Front

What does a high Rf value indicate?

Back

Strong affinity to the mobile phase.

Front

What does a low Rf value indicate?

Back

Strong affinity to the stationary phase.

Front

What role does temperature play in paper chromatography?

Back

It can affect the solubility of the components and the viscosity of the solvent.

Front

Why is it important to use pure solvents in chromatography?

Back

Impurities can interfere with the separation process and affect the accuracy of results.