Forensic Science DNA Analysis Techniques

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What is the purpose of PCR?

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The purpose of PCR (Polymerase Chain Reaction) is to amplify or copy DNA.

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Why is it important to use a new pipette tip for each sample?

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Using a new pipette tip for each sample is important to avoid contaminating the other samples.

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What does CODIS stand for?

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CODIS stands for Combined DNA Index System, a computerized database used to store DNA information.

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What does PCR stand for?

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PCR stands for Polymerase Chain Reaction.

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What is the role of DNA profiling in forensic science?

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DNA profiling is used to identify individuals based on their unique DNA patterns, helping to link suspects to crime scenes.

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What is RFLP in DNA analysis?

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RFLP stands for Restriction Fragment Length Polymorphism, a technique used to analyze the length of DNA fragments after digestion with restriction enzymes.

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How does gel electrophoresis work?

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Gel electrophoresis separates DNA fragments based on size by applying an electric current to a gel matrix.

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What is the significance of DNA evidence in criminal investigations?

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DNA evidence can provide strong links between suspects and crime scenes, often serving as crucial evidence in court.

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What are the main components of a PCR reaction?

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The main components of a PCR reaction include DNA template, primers, DNA polymerase, and nucleotides.

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What is the function of primers in PCR?

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Primers are short sequences of nucleotides that provide a starting point for DNA synthesis during PCR.