MI 4.1.2 Background Information flashcard

Front

Anything that originates from outside of the organism can be called ________

Back

exogenous

Front

Small circular pieces of non-chromosomal DNA are known as ________

Back

plasmids

Front

A ________ promoter is continuously transcribed and translated regardless of most cellular condition.

Back

constitutive

Front

In this lab, the beta-lactamase gene is controlled by a _______ promoter.

Back

constitutive

Front

Cells that can take up DNA from the environment are called ________ cells. This process typically involves the addition of chemicals and heat.

Back

competent

Front

What is the role of plasmids in genetic engineering?

Back

Plasmids are used as vectors to transfer genetic material into cells.

Front

Define a constitutive promoter.

Back

A constitutive promoter is a type of promoter that is always active, leading to continuous gene expression.

Front

What is the difference between competent cells and non-competent cells?

Back

Competent cells can take up DNA from their environment, while non-competent cells cannot.

Front

What is the significance of the beta-lactamase gene in molecular biology?

Back

The beta-lactamase gene provides resistance to antibiotics, allowing for selection of transformed cells.

Front

Explain the process of transformation in bacteria.

Back

Transformation is the uptake of foreign DNA by a bacterial cell, leading to genetic change.