BIO 1305 DNA Replication

Front

What did Chargaff discover about the percentages of nitrogenous bases in DNA?

Back

Chargaff found that in every organism, the percentages of adenine (A) equals thymine (T) and cytosine (C) equals guanine (G).

Front

What is the role of DNA polymerases in DNA replication?

Back

DNA polymerases can only add nucleotides to a free 3’ OH group during DNA replication.

Front

What type of bond joins nitrogenous bases in DNA?

Back

Nitrogenous bases are joined by hydrogen bonds.

Front

What is the Origin of Replication (Ori)?

Back

The Origin of Replication (Ori) is a special DNA sequence where DNA replication begins.

Front

In a DNA nucleotide, what does 'A' represent?

Back

In a DNA nucleotide, 'A' represents deoxyribose, which is the sugar component.

Front

What are the four nitrogenous bases found in DNA?

Back

The four nitrogenous bases in DNA are adenine (A), thymine (T), cytosine (C), and guanine (G).

Front

What is the function of helicase in DNA replication?

Back

Helicase unwinds the DNA double helix, separating the two strands to allow replication.

Front

What is the significance of the 5' and 3' ends of a DNA strand?

Back

The 5' and 3' ends indicate the directionality of the DNA strand, which is crucial for replication and transcription.

Front

What is a primer in DNA replication?

Back

A primer is a short strand of RNA that provides a starting point for DNA synthesis.

Front

What is the difference between leading and lagging strands in DNA replication?

Back

The leading strand is synthesized continuously, while the lagging strand is synthesized in short segments called Okazaki fragments.