PCR

Front

What is PCR?

Back

PCR (Polymerase Chain Reaction) is a technique used to amplify specific segments of DNA, producing millions of copies of a particular DNA sequence.

Front

What is the purpose of PCR?

Back

To produce millions of copies of a specific region of DNA.

Front

What is the first step in PCR?

Back

Denaturation.

Front

At what temperature does the Denature step of PCR occur?

Back

94-96° C.

Front

Does PCR require a primer?

Back

Yes.

Front

What is the role of primers in PCR?

Back

Primers are short sequences of nucleotides that provide a starting point for DNA synthesis during PCR.

Front

What happens during the Denaturation step of PCR?

Back

The double-stranded DNA melts open to form two single strands.

Front

What is the second step in PCR?

Back

Primer annealing.

Front

What occurs during the Primer annealing step?

Back

Primers bind to the complementary sequences on the single-stranded DNA.

Front

What is the third step in PCR?

Back

Primer extension.