Recombinant DNA

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What is recombinant DNA?

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Recombinant DNA is a form of DNA that is created by combining DNA from two different sources, often involving a plasmid and a desired trait.

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What is a plasmid?

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A plasmid is a small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently.

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What is the role of restriction enzymes in recombinant DNA technology?

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Restriction enzymes are proteins that cut DNA at specific sequences, allowing scientists to manipulate DNA for recombinant DNA creation.

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What is the first step in creating recombinant DNA?

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The first step is to cut the plasmid and the desired gene using the same restriction enzyme.

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What happens after the plasmid and desired gene are cut?

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After cutting, the desired gene is inserted into the plasmid, creating recombinant DNA.

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What is the final step in the recombinant DNA process?

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The final step is to place the recombinant DNA into bacteria for replication.

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Are restriction enzymes specific or non-specific in their action?

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Restriction enzymes are specific; they recognize and cut DNA at particular sequences.

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What is the significance of using the same restriction enzyme for both plasmid and gene?

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Using the same restriction enzyme ensures that the ends of the DNA fragments are compatible for ligation.

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Can recombinant DNA be used in medicine?

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Yes, recombinant DNA technology is used in medicine to produce insulin, vaccines, and other therapeutic proteins.

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What is gene cloning?

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Gene cloning is the process of making multiple copies of a specific gene, often using recombinant DNA technology.