What is gel electrophoresis?
Gel electrophoresis

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•
Biology
•
9th Grade
•
Hard
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1.
FLASHCARD QUESTION
Front
Back
A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.
2.
FLASHCARD QUESTION
Front
What is the principle behind gel electrophoresis?
Back
Molecules are pulled through a gel matrix by an electric field, with smaller molecules moving faster than larger ones.
3.
FLASHCARD QUESTION
Front
What is the role of agarose gel in gel electrophoresis?
Back
Agarose gel acts as a medium that allows the separation of DNA fragments based on size.
4.
FLASHCARD QUESTION
Front
How does the size of DNA fragments affect their movement in gel electrophoresis?
Back
Smaller DNA fragments move faster and travel further through the gel than larger fragments.
5.
FLASHCARD QUESTION
Front
What is the purpose of adding restriction enzymes to DNA before gel electrophoresis?
Back
Restriction enzymes cut DNA into smaller fragments, allowing for analysis of specific regions.
6.
FLASHCARD QUESTION
Front
What is a DNA ladder in gel electrophoresis?
Back
A DNA ladder is a mixture of known DNA fragments used as a reference to determine the size of unknown DNA fragments.
7.
FLASHCARD QUESTION
Front
What is the significance of the negative and positive electrodes in gel electrophoresis?
Back
DNA is negatively charged, so it moves towards the positive electrode when an electric current is applied.
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