Gel electrophoresis

Gel electrophoresis

Assessment

Flashcard

Biology

9th Grade

Hard

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15 questions

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1.

FLASHCARD QUESTION

Front

What is gel electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

2.

FLASHCARD QUESTION

Front

What is the principle behind gel electrophoresis?

Back

Molecules are pulled through a gel matrix by an electric field, with smaller molecules moving faster than larger ones.

3.

FLASHCARD QUESTION

Front

What is the role of agarose gel in gel electrophoresis?

Back

Agarose gel acts as a medium that allows the separation of DNA fragments based on size.

4.

FLASHCARD QUESTION

Front

How does the size of DNA fragments affect their movement in gel electrophoresis?

Back

Smaller DNA fragments move faster and travel further through the gel than larger fragments.

5.

FLASHCARD QUESTION

Front

What is the purpose of adding restriction enzymes to DNA before gel electrophoresis?

Back

Restriction enzymes cut DNA into smaller fragments, allowing for analysis of specific regions.

6.

FLASHCARD QUESTION

Front

What is a DNA ladder in gel electrophoresis?

Back

A DNA ladder is a mixture of known DNA fragments used as a reference to determine the size of unknown DNA fragments.

7.

FLASHCARD QUESTION

Front

What is the significance of the negative and positive electrodes in gel electrophoresis?

Back

DNA is negatively charged, so it moves towards the positive electrode when an electric current is applied.

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