Unit 1 Final Review

Front

What is most responsible for an outbreak?

Back

Pathogens

Front

PCR is used to

Back

copy DNA sequences

Front

Primers in PCR reactions are made up of

Back

single stranded DNA

Front

During the ELISA test, what happens when the enzyme attached to the secondary antibody becomes attached to the substrate?

Back

The enzyme caused the substrate to change, resulting in a color change.

Front

In what order should you add the reagents to perform an ELISA test?

Back

Antigen sample, primary antibody, enzyme-linked secondary antibody, substrate

Front

________ have surface _________ where _________ attach.

Back

pathogens, antigens, antibodies

Front

Which test best enables doctors at the CDC to identify an unknown pathogen from an outbreak?

Back

Use an automated sequencer to determine the nucleotide sequence of the samples, then compare these sequences with known organisms using BLAST.

Front

Which of the following is a medical intervention? PCR, BLAST, DNA sequencing, All of the above

Back

All of the above

Front

What career combines molecular biology and computer analysis?

Back

bioinformatics

Front

Place the following processes for using DNA for bacterial identification in order: 1. obtain bacterial DNA by bursting open bacterial cells with enzymes 2. Determine the sequence of bacterial DNA by reading the order of fluorescent nucleotides 3. Use BLAST to match the bacterial sequence to known organisms 4. Use specific primers and PCR to amplify specific pieces of bacterial DNA. 5. Use fluorescent tagged nucleotides in sequencing PCR.

Back

1, 4, 5, 2, 3