MI 2.1.2 PCR prelab flashcard

Front

The P in PCR stands for

Back

polymerase

Front

Why are two different primers needed?

Back

one for each end of the target sequence

Front

DNA polymerase always adds nucleotides in the ________ direction.

Back

5' -----> 3'

Front

Name the process of allowing Taq polymerase to add nucleotides to the new growing DNA strand.

Back

extension

Front

If the target DNA sequence is A A G T C, what complementary sequence will be added by polymerase?

Back

T T C A G

Front

Electrophoresis gel made from a seaweed-based material.

Back

agarose