MI 2.1.3 Review

Front

What is the final step in the PCR cycle?

Back

Final Extension: 72°C for 5 minutes.

Front

What is the purpose of performing a restriction digest on the PCR product?

Back

To determine if a single nucleotide polymorphism (SNP) is present at nucleotide 145.

Front

What does SNP stand for in genetics?

Back

Single Nucleotide Polymorphism.

Front

At what temperature should the restriction digest be incubated?

Back

37°C.

Front

What is the name of the thermostable polymerase used in PCR?

Back

Taq polymerase.

Front

What is the initial step in the PCR cycle?

Back

Initial denaturation: 94°C for 4 minutes.

Front

What is the denaturation temperature in PCR?

Back

94°C.

Front

What is the annealing temperature in PCR?

Back

64°C.

Front

What is the role of Taq polymerase in PCR?

Back

To synthesize new DNA strands by adding nucleotides.

Front

What is a restriction digest?

Back

A process that cuts DNA into smaller fragments using restriction enzymes.