M4.7 Gel Electrophoresis_HBio

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What is DNA extraction?

Back

The process of collecting DNA from the nucleus of a cell.

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What does gel electrophoresis do?

Back

It separates DNA fragments based on size/weight.

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Where do the largest DNA fragments appear in gel electrophoresis?

Back

Closest to the starting wells.

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What is the main purpose of gel electrophoresis?

Back

To analyze DNA by separating its molecules by size.

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What is the role of agarose gel in gel electrophoresis?

Back

It acts as a medium through which DNA fragments can move.

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What is the charge of DNA molecules?

Back

DNA molecules are negatively charged due to their phosphate backbone.

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What is the significance of the buffer solution in gel electrophoresis?

Back

It maintains the pH and provides ions that help conduct electricity.

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What is a DNA fingerprint?

Back

A unique pattern of DNA fragments that can be used for identification.

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What is the purpose of using a DNA ladder in gel electrophoresis?

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To provide a reference for estimating the size of DNA fragments.

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What is the difference between DNA extraction and gel electrophoresis?

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DNA extraction is the process of isolating DNA, while gel electrophoresis is used to separate and analyze DNA fragments.