O2 Intro to Cells and Microscopy

Front

What is the formula to calculate the field of view under different magnifications?

Back

Field of View (Low Power) x (Low Power Magnification / High Power Magnification) = Field of View (High Power)

Front

What is the actual size of an object if its image measures 450 mm at a magnification of 15,000x?

Back

Actual Size = Image Size / Magnification = 450 mm / 15,000 = 30 µm

Front

Define 'Freeze Fracture' in the context of electron microscopy.

Back

Freeze Fracture is a method of preparing samples for electron microscopy that splits the membrane along the middle of the phospholipid bilayer.

Front

What are the three main components of the cell theory?

Back

1. All living organisms are made of one or more cells. 2. Cells are the basic unit of structure and function of all living things. 3. All cells come from other living cells.

Front

If each division on a microscope scale represents 100 µm, how long is an organism that measures 4 divisions?

Back

Length of organism = 4 divisions x 100 µm/division = 400 µm.

Front

What is the purpose of using different objective lenses on a microscope?

Back

Different objective lenses provide varying levels of magnification and field of view, allowing for detailed observation of specimens.

Front

What is the significance of the cell theory in biology?

Back

The cell theory is fundamental as it establishes that all living things are composed of cells, which are the basic units of life.

Front

How do you convert millimeters to micrometers?

Back

1 mm = 1,000 µm. To convert mm to µm, multiply by 1,000.

Front

What is the magnification of a microscope if the eyepiece is 10x and the objective lens is 40x?

Back

Total Magnification = Eyepiece Magnification x Objective Lens Magnification = 10x x 40x = 400x.

Front

What is the role of the phospholipid bilayer in cell membranes?

Back

The phospholipid bilayer forms the fundamental structure of cell membranes, providing a barrier that separates the interior of the cell from the external environment.