Biotechnology Fall Final

Front

What is gene cloning?

Back

A process of isolating and making copies of a gene.

Front

What is the purpose of the polymerase chain reaction (PCR)?

Back

To make many copies of a gene or other DNA segment.

Front

Calculate the volume in mL of 50x TAE needed to make 2 L of 1x TAE electrophoresis buffer. Use C1V1=C2V2.

Back

40 mL.

Front

What is the name of this circular bacterial DNA?

Back

Plasmid.

Front

LB Broth and agar is best for which microorganism?

Back

E.coli.

Front

Define biotechnology.

Back

The use of living systems and organisms to develop or create products.

Front

What is a plasmid?

Back

A small, circular piece of DNA found in bacteria that can replicate independently.

Front

Explain the role of restriction enzymes in biotechnology.

Back

Restriction enzymes cut DNA at specific sequences, allowing for gene cloning and manipulation.

Front

What is the significance of the genetic code?

Back

It is the set of rules by which information encoded in genetic material is translated into proteins.

Front

What is transformation in the context of genetic engineering?

Back

The process of introducing foreign DNA into a host cell.