DNA Extraction Lab Prep

Front

What is DNA extraction?

Back

DNA extraction is the process of isolating DNA from cells or tissues, allowing for further analysis and experimentation.

Front

Why is it important to add ethanol slowly during DNA extraction?

Back

Adding ethanol slowly helps to prevent the DNA from shearing and ensures better precipitation.

Front

What role does salt play in DNA extraction?

Back

Salt helps to remove proteins that are chemically bound to DNA, facilitating its isolation.

Front

What is the purpose of using soap in the extraction solution?

Back

Soap dissolves the lipid bilayers of cell membranes, releasing DNA into the solution.

Front

What is the function of cheesecloth in DNA extraction?

Back

Cheesecloth is used to filter out cellular debris and separate the DNA solution from solid materials.

Front

What happens to DNA when it is mixed with ethanol?

Back

DNA precipitates out of the solution, forming visible strands.

Front

Why is it necessary to dispose of materials like cheesecloth after the lab?

Back

Used materials may contain biological contaminants and should be disposed of properly to maintain safety.

Front

What is the significance of using cold ethanol in DNA extraction?

Back

Cold ethanol enhances the precipitation of DNA, making it easier to collect.

Front

What is a common source of DNA for extraction in a lab setting?

Back

Common sources include fruits like strawberries, which have large amounts of DNA in their cells.

Front

What is the role of the buffer solution in DNA extraction?

Back

The buffer solution maintains the pH and provides the necessary ions for the extraction process.