MBT Quarter 2 Review

Front

What is heat shock in cellular biology?

Back

Heat shock refers to a process that enlarges the pores of the cell membrane, allowing for the uptake of plasmids and other molecules.

Front

What type of chromatography is used to separate proteins based on their size?

Back

Size Exclusion Chromatography is used to separate proteins based on their size, allowing smaller molecules to pass through while larger ones are retained.

Front

What is the purpose of the Extension step in PCR?

Back

The Extension step of PCR allows polymerase to create the complementary strands of DNA.

Front

How does the human immune system respond to invading organisms?

Back

The immune system responds by producing antibodies that target the epitopes on the surface of the invading organism.

Front

What is the mobile phase in chromatography?

Back

The mobile phase is the buffer used in chromatography that carries the sample through the stationary phase.

Front

Define plasmid.

Back

A plasmid is a small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently.

Front

What is the role of polymerase in DNA replication?

Back

Polymerase synthesizes new DNA strands by adding nucleotides complementary to the template strand.

Front

What is the function of antibodies in the immune response?

Back

Antibodies bind to specific antigens on pathogens, neutralizing them and marking them for destruction by other immune cells.

Front

Explain the concept of size exclusion in chromatography.

Back

Size exclusion chromatography separates molecules based on their size, allowing larger molecules to elute first while smaller ones are retained longer.

Front

What is the significance of the stationary phase in chromatography?

Back

The stationary phase is the solid or viscous material that interacts with the sample, allowing for separation based on different affinities.