MI4.1.2 Modules 2&3 Pre-Lab

Front

What is the effect of positioning a tube on its side when using lysis buffer?

Back

Cells with lysis buffer will freeze faster.

Front

Which plate is used to make a seed culture in Module 1?

Back

+DNA/+Amp/+IPTG

Front

Which of the following is NOT a method to lyse cells?

Back

Vortexing is NOT a method meant to help lyse the cells.

Front

How many colonies should be picked with the loop for a seed culture?

Back

4-5 colonies should be picked.

Front

What does it indicate if the pellet is fluorescent instead of the supernatant?

Back

It indicates that the cells are still intact.

Front

Define lysis buffer.

Back

A lysis buffer is a solution used to break down cell membranes to release cellular contents.

Front

What is the purpose of a hot water bath in cell lysis?

Back

A hot water bath helps to denature proteins and facilitate cell lysis.

Front

Explain the role of IPTG in bacterial cultures.

Back

IPTG is an inducer used to promote the expression of genes in bacterial cultures.

Front

What is the significance of using antibiotics like Ampicillin in culture plates?

Back

Ampicillin is used to select for bacteria that have taken up a plasmid containing the antibiotic resistance gene.

Front

What does a fluorescent pellet indicate in a cell lysis experiment?

Back

A fluorescent pellet indicates that the cells have not lysed and are still intact.