Thin Layer Chromatography

Front

What is Thin Layer Chromatography (TLC)?

Back

A technique used to separate non-volatile mixtures based on their different affinities to a stationary phase and a mobile phase.

Front

What does Rf stand for in chromatography?

Back

Rf stands for 'retention factor', which is a ratio used to characterize the movement of compounds on a chromatographic medium.

Front

How is Rf calculated?

Back

Rf = distance travelled by sample / distance travelled by solvent.

Front

What factors affect the distance travelled by a compound in TLC?

Back

The distance travelled depends on the compound's adherence to the plate and its solubility in the solvent.

Front

What is the mobile phase in paper chromatography?

Back

The mobile phase is typically a liquid that carries the sample along the stationary phase.

Front

What is the stationary phase in Thin Layer Chromatography?

Back

The stationary phase is a solid or viscous liquid that remains fixed on the plate and interacts with the compounds.

Front

What role does solvent polarity play in TLC?

Back

Solvent polarity affects the solubility of compounds, influencing their movement and separation on the chromatographic plate.

Front

What is the significance of the Rf value?

Back

The Rf value helps identify compounds by comparing their movement in a specific solvent system.

Front

Which factor does NOT affect the Rf value?

Back

Time taken does not affect the Rf value; it is determined by the solvent and the compound's properties.

Front

What is the purpose of using a TLC plate?

Back

A TLC plate is used to separate and analyze compounds in a mixture based on their different affinities.