
12/27 - Laboratory Techniques
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Biology
•
University
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Practice Problem
•
Hard
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1.
FLASHCARD QUESTION
Front
What is a restriction enzyme?
Back
A restriction enzyme is a protein that cuts DNA at specific sequences, known as recognition sites, allowing for DNA manipulation in molecular biology.
2.
FLASHCARD QUESTION
Front
What is column chromatography?
Back
Column chromatography is a laboratory technique used to separate mixtures based on the differential affinities of compounds to the stationary phase (e.g., glass beads) and the mobile phase.
3.
FLASHCARD QUESTION
Front
What does it mean for a compound to be polar?
Back
A polar compound has a distribution of electrical charge that leads to the formation of positive and negative poles, resulting in interactions with other polar substances.
4.
FLASHCARD QUESTION
Front
What is isoelectric focusing?
Back
Isoelectric focusing is a technique used to separate proteins based on their isoelectric point (pI), where proteins migrate in a pH gradient until they reach a point where their net charge is zero.
5.
FLASHCARD QUESTION
Front
What is the significance of a protein's isoelectric point (pI)?
Back
The isoelectric point (pI) is the pH at which a protein has no net charge, affecting its solubility and interaction with other molecules.
6.
FLASHCARD QUESTION
Front
What is UV-Vis spectrometry used for?
Back
UV-Vis spectrometry is used to analyze the absorbance of UV and visible light by a sample, helping to identify the presence of conjugated pi systems and functional groups.
7.
FLASHCARD QUESTION
Front
What is the difference between simple and fractional distillation?
Back
Simple distillation is used for separating liquids with significantly different boiling points (usually >25°C), while fractional distillation is used for separating liquids with closer boiling points (typically <25°C).
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