
ABE Chapter 4 Glossary
Flashcard
•
Science
•
11th Grade
•
Practice Problem
•
Easy
Arlyssa Porte
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8 questions
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1.
FLASHCARD QUESTION
Front
DNA ladder
Back
A set of known DNA fragments with different sizes in base pairs (bp) or kilo bases (kb). These DNA fragments are separated and visualized as DNA bands on a gel. Together, the separated DNA bands look like a ladder on the gel. Used in gel electrophoresis to determine the size and quantity of unknown DNA fragments.
2.
FLASHCARD QUESTION
Front
Ligate
Back
To join together two DNA ends.
3.
FLASHCARD QUESTION
Front
Ligation
Back
The reaction that chemically joins two or more fragments of DNA, resulting in a recombinant DNA molecule.
4.
FLASHCARD QUESTION
Front
Loading Dye
Back
A set of dyes that are added to biomolecules such as DNA for gel electrophoresis. One dye moves farther than the sample, which indicates that it is time to stop running the gel.
5.
FLASHCARD QUESTION
Front
Multimer
Back
A plasmid configuration consisting of multiple plasmids that have interlocked during formation so they are like links in a chain.
6.
FLASHCARD QUESTION
Front
Nicked Circle
Back
A plasmid configuration that consists of a single plasmid that has a break in one of its two strands. The shape of this plasmid is circular.
7.
FLASHCARD QUESTION
Front
Supercoiled
Back
A plasmid configuration consisting of a single plasmid that has been twisted. The shape of this plasmid is more compact (takes up less space) than the circular form.
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