BACE: Biotechnology Skills

Front

1. 1 How do you accurately measure liquids using micropipettes and serological pipets?

Back

Use a micropipette for very small liquid volumes and a serological pipet for larger amounts. Always set the correct volume, hold the pipette at the right angle, and release liquid smoothly.

Front

2 How do you accurately measure mass using an electronic balance?

Back

Turn on the balance, make sure it reads zero, place the object or container on the scale, and record the weight.

Front

3 How do you properly prepare solutions and buffers?

Back

Measure the correct amounts of chemicals and mix them in water while following a recipe. Use a pH meter to check the acidity or basicity if needed.

Front

4 How do you measure and adjust the pH of a solution with a pH meter?

Back

Use a pH meter to check how acidic or basic a solution is, then add acid or base to adjust it.

Front

5 How do you properly perform a serial dilution?

Back

Mix a known amount of liquid with a new solution multiple times to gradually reduce its concentration.

Front

6 How do you identify and properly handle reagents?

Back

Read labels carefully, store them correctly, and use protective equipment if needed.

Front

7 What is aseptic or sterile technique?

Back

Aseptic technique means working in a way that prevents contamination by bacteria or other germs.

Front

8 How do you properly culture microorganisms?

Back

Provide them with food, warmth, and the right environment to grow in a lab.

Front

9 How do you properly use a centrifuge?

Back

Balance the tubes, close the lid, set the speed, and turn it on to spin samples at high speed.

Front

10 How do you properly use a spectrophotometer?

Back

Place a sample in the machine, select the right settings, and measure how much light passes through the liquid.