PCR Flashcard

Front

What is PCR?

Back

PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA sequences, making millions of copies of a particular segment of DNA.

Front

What happens in the Denature step of PCR?

Back

The double-stranded DNA is separated into two single strands of DNA.

Front

At what temperature does the Denature step of PCR occur?

Back

94-96° C.

Front

What is the purpose of the Denature step in PCR?

Back

To separate the double-stranded DNA into single strands, allowing access for primers.

Front

What happens in the Annealing step of PCR?

Back

Primers bind to the single-stranded DNA templates.

Front

At what temperature does the Annealing step of PCR occur?

Back

50-65° C.

Front

What is the purpose of the Annealing step in PCR?

Back

To allow primers to attach to the complementary sequences on the single-stranded DNA.

Front

What happens in the Extension step of PCR?

Back

Taq polymerase synthesizes new DNA strands by adding nucleotides to the primers.

Front

At what temperature does the Extension step of PCR occur?

Back

72° C.

Front

What is the role of Taq polymerase in PCR?

Back

Taq polymerase is an enzyme that synthesizes new DNA strands during the Extension step.