NGS Flashcard

Front

Which generation of sequencing is NOT mentioned in the outline? Options: First generation, Second generation, Third generation, Fourth generation

Back

First generation

Front

Which of the following is an application of NGS related to DNA-protein interactions? RNA-Seq, ChIP-Seq, Metagenomics, Microbiome study

Back

ChIP-Seq

Front

In what year was the structure of DNA solved?

Back

1953

Front

Who developed the first genome sequencing method in 1977?

Back

Sanger

Front

When was the Human Genome Project completed?

Back

2003

Front

What was the first bacterial genome sequenced in 1995?

Back

H. influenzae

Front

What DNA sequencing method was introduced around 1980?

Back

Sanger method

Front

What is the role of polymerase in Sanger sequencing?

Back

To elongate DNA sequences

Front

What causes the enzyme to stop at a random position in Sanger sequencing?

Back

ddNTP

Front

How are terminated copies separated in Sanger sequencing?

Back

By size in a gel