Gram Staining Procedure Steps

Front

What is the first step in the Gram staining procedure?

Back

Label four transfer pipets.

Front

What is the purpose of Gram staining?

Back

To differentiate between Gram-positive and Gram-negative bacteria based on their cell wall composition.

Front

What should you do if the isolation streak was unsuccessful?

Back

Consult your instructor.

Front

What is the role of Gram's Crystal Violet in the Gram staining procedure?

Back

It is the primary stain that colors all cells initially.

Front

What is the next step after applying the primary stain in Gram staining?

Back

Rinse with distilled water.

Front

What is the purpose of the decolorization step in Gram staining?

Back

To remove the primary stain from Gram-negative bacteria.

Front

What should you do if the flame does not touch the central section of the slide?

Back

Continue as normal.

Front

What is the purpose of applying a counterstain in Gram staining?

Back

To stain the decolorized Gram-negative bacteria, making them visible.

Front

What should you do after spreading the mixture within the circle on the slide?

Back

Incubate the slide.

Front

What is the significance of repeating steps 6-8 for the second strain of bacteria?

Back

To compare results.