Electrophoresis Lab PreFlashcard

Front

What is electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What is the purpose of using a low intensity blue light in electrophoresis?

Back

To help visualize the wells when loading samples.

Front

Where should the power supply be connected in the MiniOne® Carriage?

Back

The power supply should be plugged into the back of the carriage.

Front

How frequently should migration of bands be checked during electrophoresis?

Back

Every 5 minutes.

Front

What should be avoided until the gel solidifies?

Back

Disturbing the gel.

Front

What is the standard volume of each sample loaded into the lanes during electrophoresis?

Back

10 µL.

Front

What is agarose gel used for in electrophoresis?

Back

To create a medium through which DNA or proteins can migrate.

Front

What is the role of the comb in gel electrophoresis?

Back

To create wells in the gel for sample loading.

Front

What happens to DNA fragments during electrophoresis?

Back

They migrate towards the positive electrode due to their negative charge.

Front

What is the significance of the buffer solution in electrophoresis?

Back

It maintains pH and provides ions that carry the current.