U3 AOS 1: Recombination and transformation

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Plasmid

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A small, circular piece of DNA found in bacteria, used as a vector in genetic engineering.

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Recombinant plasmid

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A plasmid that has been genetically modified to include a gene of interest.

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Bacterial transformation

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The process of introducing foreign DNA into a bacterial cell.

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Genetic modification

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The alteration of the genetic material of an organism to achieve desired traits.

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Cloning vector

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A DNA molecule used to carry foreign genetic material into a cell.

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Gene of interest

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The specific gene that is being studied or manipulated in genetic engineering.

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Restriction endonuclease site

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A specific sequence of DNA where restriction enzymes cut.

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Antibiotic resistance gene

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A gene that provides resistance to antibiotics, used as a marker in genetic engineering.

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Origin of replication (ORI)

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A sequence in a genome where replication is initiated.

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Reporter gene

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A gene used to indicate if a certain gene has been taken up by or expressed in the cell.