MI EOC Review

Front

What is the correct order of reagents to perform an ELISA test?

Back

1. Antigen sample 2. Primary antibody 3. Enzyme-linked secondary antibody 4. Substrate

Front

What is a restriction enzyme?

Back

An enzyme that cuts DNA molecules at specific recognition sites.

Front

What is the purpose of using an automated sequencer in identifying pathogens?

Back

To determine the nucleotide sequence of the samples and compare these sequences with known organisms using BLAST.

Front

What type of cancer is osteosarcoma?

Back

Bone cancer.

Front

Where is the auditory nerve located in a diagram?

Back

Position I.

Front

What is the function of a primary antibody in an ELISA test?

Back

To specifically bind to the target antigen.

Front

What is PCR and its role in pathogen identification?

Back

Polymerase Chain Reaction (PCR) amplifies specific DNA sequences, making it easier to analyze.

Front

What does BLAST stand for in genetic analysis?

Back

Basic Local Alignment Search Tool.

Front

What is the role of the enzyme-linked secondary antibody in an ELISA test?

Back

To bind to the primary antibody and produce a measurable signal.

Front

What is the significance of a recognition site for restriction enzymes?

Back

It is a specific sequence of nucleotides where the enzyme cuts the DNA.