Microbial Metabolism

Microbial Metabolism Homework

Objective: Review Ch. 7 and Check Comprehension

How Does This Work?

• Examine the figures or watch the videos

• Look at the question on the slide

• It would be a good idea to have your notes handy

• Consulting the module pages or class slides is also a good idea

• Answer the question on the slide

• Repeat

Overview of Glycolysis

image from Khan Academy

Multiple Choice

Which of these processes represents anabolism?

• Pyruvate is made from glucose

• Ethanol is produced from glucose

• Peptidoglycan is made from amino acids and CHO

• Pyruvate is converted into carbon dioxide

Multiple Select

Select ALL the events that occur in glycolysis:

• Glucose is produced

• NADH is produced

• ATP is produced

• Acetyl coA is produced

Multiple Choice

Which form of the electron carrier below can accept electrons and become reduced?

• NAD+

• NADH

Need a refresher? Rewatch the animation on the next slide.

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Multiple Choice

Which form of the electron carrier is produced in glycolysis and the citric acid cycle?

• NAD+

• NADH

Multiple Choice

In glycolysis, Glucose is _____ to Pyruvate.

• Oxidized

• Reduced

Multiple Choice

In this reaction, which molecule(s) is/are BEING oxidized?

NAD⁺ + glyceraldehyde-3-P --> NADH + 1,3 bisphosphoglycerate

• NAD+

• NADH

• glyceraldehyde-3-P

• 1,3 bisphosphoglycerate

The Link Reaction

• The video on the next slide explains the connecting reaction between glycolysis and the citric acid cycle

• Don't dwell on the details such as the enzyme name and coA

Multiple Choice

True or False? Pyruvate is oxidized (and decarboxylated) to produce acetyl coA. [Yes, I know, I'm still asking oxidation-reduction questions.]

• True

• False

Citric Acid/Krebs/TCA Cycle

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Multiple Choice

In the Citric Acid Cycle, acetyl coA is

• reduced to pyruvate

• oxidized to CO₂

• used to synthesize fatty acids

• converted into ATP

Multiple Select

The Citric Acid Cycle produces (select ALL that are correct):

• acetyl coA

• 2 ATP/GTP

• 6 NADH

• H⁺ gradient

• pyruvate

Electron Transport Chain & ATP Synthase

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Energy Conversions

Remember the C in glucose has been oxidized to CO₂ so the ETC is not acting on C-containing substrates such as glucose, acetyl coA or pyruvate. The energy is now in the form of NADH and we want to use it to make ATP.

Multiple Choice

The source of energy for the ETC is:

• ATP

• O₂ to H₂O

• protons in NADH

• electrons in NADH

Multiple Choice

The electron carriers of the ETC create energy that is now in the form of:

• NAD⁺

• ATP

• H⁺ gradient

• O₂ to H₂O

Multiple Choice

The ATP synthase uses which form of energy to produce ATP?

• ADP + Pi

• H⁺ gradient

• NAD⁺

• NADH

Fermentation

image from Khan Academy

Multiple Select

Fermenting cells utilize which of the following pathways/processes? (Select all that apply)

• Glycolysis

• Citric Acid Cycle

• ETC/ATP Synthase

• Anaerobic respiration

Multiple Choice

Like Glycolysis and Citric Acid Cycle, Fermentation reactions also produce NADH.

• True

• False

Multiple Choice

True or False? If oxygen is present, cells cannot ferment.

• True

• False