TRANSCRIPTION

TRANSCRIPTION

TRANSCRIPTION HAS 3 STEP

• Initiation

• Elongation

• Termination

INITIATION

• Transcription factor recognise the promoter region or TATA box & bind to the promoter.

• RNA polymerase binds to transcription factor (at promoter) to create Transcription Initiation Complex.

• RNA polymerase then starts transcription.

Multiple Choice

What is the template used in transcription?

• DNA strand

• mRNA strand

• tRNA strand

Multiple Choice

What enzyme does transcription use?

• DNA polymerase

• ligase

• RNA polymerase

• helicase

ELONGATION

• RNA polymerase adds RNA nucleotides to the 3^' end of the growing RNA molecule as it continues along the double helix.

• The new RNA molecule peels away from its DNA template & the DNA double helix reforms.

Multiple Choice

Which RNA bases would pair with TACGAA in transcription?

• AUGCUU

• ATGCTT

• GCATCC

• GCAUCC

Multiple Choice

Which of these bases does not exist in RNA?

• C

• T

• U

• A

Multiple Choice

One difference between DNA and RNA is that....

• Only RNA has the base Uracil

• only RNA contains the base Thymine

• only RNA contains the base Cytocine

• only RNA contained the base Guanine

TERMINATION

• The RNA polymerase continue to transcribing for hundreds of nucleotide past the terminator sequence, AAUAAA.

• At a point about 10 to 35 nucleotide past this sequence ( AAUAAA), the pre-mRNA is cut from the RNA polymerase.

Multiple Choice

What is the product form transcription?

• rRNA

• tRNA

• mRNA

• DNA strand

Multiple Choice

what does mRNA do?

• carry info from genes to ribosomes

• carry amino acids to ribosomes

• help with RNA splicing

• make up ribosomes

Multiple Choice

Where does transcription take place?

• nucleus

• ribosome

• cytoplasm

• smooth ER