Biochemical Techniques

Biochemical Techniques

Multiple Choice

Chromatography is generally used for the separation of mixtures based on the differences in their

• shape

• size

• structure

• all of the above

Multiple Choice

Ion-exchange chromatography is used for the separation of

• polar molecules

• nonpolar molecules

• none of the above

• all of the above

Multiple Choice

Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?

• ion exchange chromatography

• affinity chromatography

• adsorption chromatography

• gel permeation chromatography

Multiple Choice

The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

• V = V0 + kDVi

• V = V0/Vi

• V = V0 – kDVi

• none of the above

Multiple Choice

In gel filtration chromatography, separation of proteins are based on their

A size and net charge

B size and shape

C size and specific affinity

D shape and net charge

• size and net charge

• size and shape

• size and specific affinity

• shape and net charge

Multiple Choice

Protein separation techniques are often based on the following properties except

• solubility of the protein

• viscosity of the protein

• charge of protein

• specific binding affinity of the protein

Multiple Choice

Which polymer is often used for matrix materials in Affinity chromatography?

• Agarose

• cellulose

• dextrose

• all of the above

Multiple Choice

In anion exchange chromatography,

• the column contains negatively charged beads where positively charged proteins bind

• the column contains positively charged beads where negatively charged proteins bind

• the column contains negatively & positively charged beads where proteins bind depending on their net charge

• all of the above

Multiple Choice

In SDS -PAGE , proteins are separated on the basis of their

• shape

• size

• charge

• all of the above