MPA 9 Effect of temperature on enzyme action

MPA 9 Effect of temperature on enzyme action

The enzyme catalase acts on the substrate hydrogen peroxide and produces water and oxygen. Yeast solution or celery juice can be used as a source of catalase.

The pH is kept constant throughout the experiment by using pH 7 buffer. Mix together chopped celery and water and place in a test tube in a water bath at 25 degrees C. Place Hydrogen peroxide in a graduated cylinder with pH 7 buffer in the water bath. Leave both solutions for 10 minutes. 

Add the celery extract solution to the hydrogen peroxide and record the volume of foam produced by oxygen after 10 seconds as a measure of the reaction rate. Repeat the experiment at different temperatures using different waterbaths. 

The optimum temperature produces the largest volume of foam in the same time. At higher temperatures the enzyme is denatured and at lower temperatures the enzyme moves slowly and does not collide with the substrate with the same frequency.

Multiple Choice

Enzyme are made out of ________________.

• carbohydrates

• lipids

• nucleic acids

• protein

Multiple Choice

______________ are catalysts for chemical reactions in living things.

• Acids

• Bases

• Substrates

• Enzymes

Multiple Choice

Changes in pH and temperature can damage an enzymes active site.  What is this called?

• reusable
• catalyst
• specific
• denature

Multiple Choice

The pH at which an enzyme is most efficient is called the _________ pH of an enzyme.

• neutral

• optimum

• minimum

• experimental

Multiple Choice

Name the enzyme used in this investigation.

• starch

• protein

• celery

• catalase

Multiple Choice

Name the substrate for the enzyme catalase

• starch

• protein

• celery

• hydrogen peroxide

Multiple Choice

Name the products of the action of catalase

• starch and glucose

• protein and amino acids

• Water and oxygen

• carbon dioxide and maltosse

Multiple Choice

How is the pH of the enzyme kept constant.

• same pH bath

• same pH buffer

• different pH buffer

• pH 7

Multiple Choice

How is the temperature of the enzyme varied

• same water bath

• Different water bath

• 37 degrees C

• 25 degrees C

Multiple Choice

How is the rate of catalase action measured?

• Volume of water produced

• Volume of oxygen produced

• Height of oxygen foam produced in a fixed time.

• Volume of water produced in a fixed time