Earl Washington Case

Multiple Select

Was Earl Washington guilty of raping and murdering Rebecca Williams?

• yes

• no

Multiple Select

In your Opinion: Did the criminal justice system work as it should have?

• yes

• no

Open Ended

What biological specimens would yield DNA at a crime scene? List examples!

Multiple Choice

Which of the following materials are not needed to perform a PCR reaction?

• DNA polymerase

• RNA template

• DNA template

• free nucleotides

• primers

Multiple Choice

You set up a PCR reaction using a human DNA polymerase enzyme and the other required materials. What results will you observe after running the PCR for 30 cycles?

• The PCR will produce > 1 billion copies of DNA

• The PCR will produce > 1 million copies of DNA

• The PCR will produce only one copy of DNA

• The PCR will not produce any copies of DNA

Multiple Choice

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

Multiple Choice

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

Multiple Choice

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

Multiple Choice

The first step in PCR is

• Primer annealing

• Denaturation

• Primer extension

• None of these

Multiple Choice

What is the denaturation step?

• mutating RNA polymerase

• Cooling DNA to allow primers to form

• separating DNA helix in a cold environment

• separating DNA helix with using heat

Match

Match the following:

• Denaturing

• Annealing

• Extension

Separation of DNA strands

Binding of primers

DNA synthesis

• Separation of DNA strands

• Binding of primers

• DNA synthesis

Drag and Drop

PCR is a ​

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used in the ​

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to make ​

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of copies of a particular section of ​

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Drag these tiles and drop them in the correct blank above

lab

millions

DNA

technique

PRC

technology

100s

Multiple Choice

If DNA was loaded at the top of a gel but the positive and negative poles were accidentally reversed, what would happen?

• All of the DNA molecules would move towards the top of the gel.

• All of the DNA molecules would move towards the bottom of the gel.

• Small DNA molecules would move towards the top of the gel and large DNA molecules would move towards the bottom of the gel.

Multiple Choice

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

Drag and Drop

To make a DNA Fingerprint, you use ​ (a)​ ​ ​

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  to cut a DNA sample into fragments, and then use ​ (b) ​

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_   to separate the fragments by size

Drag these tiles and drop them in the correct blank above

restriction enzymes

Gel Electrophoresis

Match

Match the following:

• PCR

• Gel electrophoresis

• DNA fingerprint

• Bacterial transformation

amplification of a DNA or protein sample for analysis

used to separate fragments of DNA or protein

pattern of DNA fragments used to identify an individual

taking up foreign DNA by a bacterium

• amplification of a DNA or protein sample for analysis

• used to separate fragments of DNA or protein

• pattern of DNA fragments used to identify an individual

• taking up foreign DNA by a bacterium

Multiple Choice

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Multiple Choice

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

Hotspot

The sample on the left comes from a crime scene. The three samples on the right came from suspects. Click on the DNA fingerprint of the suspect that matches the sample.

Multiple Choice

A single STR may differ between two unrelated people by…

• sequence

• length

• location in the genome

• sequence and length

Multiple Choice

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3