Enzyme Immobilization

Enzyme Immobilization

Restriction of enzyme mobility in a fixed space

Advantages of enzyme immobilization

• Enzyme reutilization

• Elimination of enzyme recovery and purification processes

• Provide a better environment for enzyme activity

• Reduce effluent handling problems

• Improve purity of enzyme

Matrix Entrapment

Enzyme Immobilization: Entrapment

Open Ended

Define in your own words what is matrix entrapment

Multiple Choice

Are matrix entrapments normally used for polymeric materials?

• True

• False

Multiple Select

Select ALL the materials that are commonly used as matrices for entrapment

• Cellulose Triacetate

• Water

• Agar

• Alginate

Multiple Choice

Which of the following is NOT an advantage for matrix entrapment?

• Low Cost

• Easy handling

• Can be practised at a large scale

• Less chance of conformational changes in enzyme

Membrane Entrapment

Enzyme Immobilization: Entrapment

Open Ended

Define in your own words what is membrane entrapment

Multiple Choice

Which of the following shows membrane entrapment?

Multiple Choice

Which of the following is the advantage of membrane entrapment?

• No diffusion barrier

• Low leakage

• Continuous product formation

Multiple Choice

Which of the following is the disadvantage of membrane entrapment?

• Catalytic activity is unaffected by chemical reaction

• Diffusional limitation

• Larger surface area for reaction

Poll

Can chemical reaction interfere the enzyme activity?

• Yes

• No

Adsorption & Covalent Binding

Enzyme Immobilization: Bound

Multiple Choice

Which of the following diagram represents a covalent-bounded enzyme immobilization?

Multiple Choice

Which of the following is considered as a disadvantage to the adsorption method of immobilization?

• It is possible to separate and purify the enzymes while being immobilized

• The enzymes are not usually deactivated by adsorption

• The adsorption is a reversible process

• State of immobilization is very sensitive to solution pH, ionic strength and

  temperature

Multiple Choice

Below are the advantages of adsorption in immobilised enzyme systems, except:

• No desorption problem

• Easy to carry out

• No reagents required

• Cheap method

Experimental Method for Immobilizing Enzymes