Recombinant DNA

Recombinant DNA

Ms. Cabrera

Multiple Choice

The main goal of the Human Genome Project (HGP) was to:

• Identify the unique parts of each person's DNA.
• Identify genes responsible for human diseases.
• Identify genes responsible for human behavior.
• Determine the entire sequence of human DNA.

Multiple Choice

What is DNA sequencing?

• The total of genes carried by an individual or cell
• Coding genes in order to identify single nucleotide polymorphisms
• Deciphering the functions of various parts of the human genome
• The process of identifying the order of the bases in DNA

Multiple Choice

Which of the following accurately describe the steps of recombinant DNA technology using bacteria?

• plasmid & genes are cut --> cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected
• cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected
• plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected-->cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria
• cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> bacteria & rDNA plasmid multiply 

Multiple Choice

Which of the following is an enzymatic protein that acts as DNA glue?

• plasmid
• restriction enzyme
• ligase
• protein expression

Multiple Choice

Which of the following is a protein that acts as DNA scissors?

• plasmid
• restriction enzyme
• ligase
• protein expression

Multiple Choice

Which of the following is a circular DNA from bacteria that can hold a foreign gene?

• plasmid
• restriction enzyme
• ligase
• protein expression