Lecture note Topic 8 Recombinant DNA Technology

Topic 8: Recombinant DNA Technology

by SALIZA BM

Multiple Select

Explain the characteristics of plasmid.

• Able to accept foreign DNA in MCS (multiple cloning sites)

• Able to replicate freely in the host cell because of the presence of origin of replication, ori

• Possess selectable marker gene (resistance to antibiotic) e.g.: ampR

• Able to express or amplify the cloned gene under suitable conditions

Multiple Select

Explain characteristics of host cell.

 

• Able to receive the recombinant DNA through transformation.

• Able to maintain the structure of the recombinant DNA from one generation to another.

• Able to amplify the gene product from recombinant DNA

• Able to express the gene of interest / cloned gene.

Multiple Choice

Area of study involves modifying the DNA of an organism to produce new genes with new traits.

• Genetic technology

• Genetic engineering / recombinant DNA technology

• Recombinant DNA engineering

• Genetic recombinant DNA / engineering technology

Multiple Choice

The base sequence of one strand reads the same as its complement when both are read in the 5’ à 3’ direction.

• Palindromic sequence

• Opposite sequence

• Normal sequence

• Template sequence

Multiple Choice

Small circular DNA molecule that maybe present and replicate inside a bacterial cell, such as E. coli.

• Host cell

• Bacteria

• Vector

• Plasmid

Multiple Choice

A molecule of DNA that has foreign DNA spliced into it.

• Cloning vector

• Recombinant DNA / plasmid 

• Host cell

• Plasmid

Multiple Choice

Covalently links two DNA fragments to form a stable recombinant DNA molecule.

• DNA polymerase

• Reverse transcriptase

• DNA cloning vector

• DNA ligase

Multiple Choice

Combination vectors with features from both bacteriophages and plasmids.

• Bacteriophages

• Cosmids

• Plasmids

• YAC

Multiple Choice

Copies of mature mRNA from which introns have been removed.

• rRNA

• RNA transcript

• Complementary DNA / cDNA

• pre-mRNA

Multiple Select

DNA molecule that can carry foreign DNA into a host cell and replicate there.

• Cloning vector

• Bacteria

• Host cell

• Recombinant plasmid

Multiple Choice

A process useful to make many copies of a particular gene and to produce a protein product.

• Cloning / Gene cloning

• Transformation

• Ligation

• Insertion

Multiple Choice

Enzyme that catalyses the formation of DNA using single stranded mRNA.

• Restriction endonuclease

• DNA polymerase

• DNA ligase

• Reverse transcriptase

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