CCU2 - Green Chem Lab

​Supplies:

-->Learning Log

-->Packet (pg 11)

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Write down the 12 Principles​

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Learning Log

Intro to green chemistry​

AKA:

Sustainable Chemistry​

​Agenda:

1. List the 12 principles of Green / Sustainable Chemistry

2. Watch and take notes on 2 videos about principles 1 & 2

3. Pre-lab questions (done in notebook)

4. Read through procedures (add notes to packet from the slides)

   1. You DO NOT need to write your procedures down

5. Create Data Table / List

6. Questions on procedures & Data Table

Subject | Subject

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Write down the 12 Principles​

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Learning Log

Multiple Choice

Environmental benefits of green chemistry include?

• Fewer raw materials and natural resources used

• Cleaner production technologies & reduced emissions

• Smaller quantities of hazardous waste to be treated and disposed of

• All of the above

Background- lab

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You may have noticed that if your town is expecting a snow or ice storm, trucks will go out and treat the roads in your neighborhood to stop the snow and ice from adhering to them. But what is that substance that is used to coat the road and does it meet the criteria for green chemistry? An effective deicer will work well on the roads, be cost effective and create little to no environmental impact. In this lab, you will test the toxicity of several concentrations of road deicers on germinating seeds and decide which road deicer is acceptable.

​Day 1- Solution Preparation

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• On day 1, your group will use a volumetric flask and a scale to create a 0.8M solution of either Sodium Chloride, Calcium Chloride or Magnesium Chloride.

• The following slides guide you through the calculations​

​Pre-lab Questions- Answer in your NB

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​Steps for making 50 ml of 1 M solutions

​

1. Calculate the molar masses​

• What is the molar mass of NaCl?

• What is the molar mass of CaCl₂?

• What is the molar mass of MgCl₂?​

Multiple Choice

What is the molar mass of Calcium Chloride?

CaCl₂

• 111 g

• 132 g

• 75 g

​Pre-lab Questions- Answer in your NB

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​Steps for making 50 ml of 0.8 M solutions

2. Use the two step method to determine the number of grams needed to make 50ml of 0.8M NaCl solution

   a. Convert 50ml to L and solve using the molarity equation (Solve for n)

Multiple Choice

How many moles are need to make 50 ml of 0.8 M NaCl solution

• 50

• 0.016

• 0.5

• 0.04

• 0.8

​Pre-lab Questions- Answer in your NB

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​2.Use the two step method to determine the number of grams needed to make 50ml of 0.8M NaCl solution

b. Use the molar mass to convert the number of moles (from part A) to grams of NaCl.

​We will discuss how to mix up a solution during class but know that we will be using a volumetric flask

Multiple Choice

How many grams of NaCl are needed to make 50 ml 0.8M solution

• 2.32 g

• 58 g

• 0.04 g

• 4.82 g

Drag and Drop

Use the dimensional analysis method to determine the number of grams of Magnesium Chloride required

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Drag these tiles and drop them in the correct blank above

Multiple Choice

3. How many grams of Calcium Chloride are required?

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• 3.0 grams

• 4.4 grams

•  0.04 grams

• 3.8 grams

4. Choose what method you want (2 step or dimensional analysis) to solve for the number of grams required to make 50ml of Calcium Chloride solution.

Multiple Choice

How many grams are required to make 50ml of Calcium Chloride solution.

• 4.44 grams

• 5.55 grams

• 6.94 grams

Multiple Choice

Ion Concentration (look at the formula of the compound)

What is the [Ca²⁺] in 0.8M CaCl₂

• 0.8 M

• 0.4M

• 1.6M

• 2.8M

Multiple Choice

Ion Concentration (look at the formula of the compound)

What is the [Cl^-] in 0.8M CaCl₂

• 0.8 M

• 0.4M

• 1.6M

• 2.8M

Multiple Choice

Name the item

• Erlenmeyer Flask

• Volumetric Flask

• Beaker

• Test Tube

Multiple Choice

Name the item

• Erlenmeyer Flask

• Volumetric Flask

• Beaker

• Test Tube

Read through the following direction & underline or highlight in your packet. Add the notes-

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​1. Label 4  additional test tubes with B-E

2. Using a graduated cylinder, add 9ml of distilled water to test tubes B-E

3. Remove 1ml of your stock solution from test tube A and add to test tube 2 and mix. Test tube B  is now a ____ M solution.

4. Using serial dilution, complete 1/10 dilutions from tubes B to E. 

• In other words, to make the 0.008 M solution mix 1 mL of ____M solution with 9 mL of water. 

5. Label a spot late with group names. Place a filter paper into the bottom of each well. Put 2 ml of each test tube solution into its associated (labelled) well.

6. Add approximately 5-6  seeds to well plate

7. On cart at the front of the classroom.

8. Allow seeds to germinate over 5 to 7 days. Be sure to check on the germinating seeds  if possible.

9. Record your observations in the data table.​

Day 2:Preparing ________ (You will Seeds for Germination:

0.8

0.08

Note: Break up the tasks
Team 1: Measure out 9ml of DI water; complete the serial dilutions
Team 2: Add filter paper and seeds to well plate. Add Solutions to well Plate

Obtaining data after one week:

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1. Count the number of seeds germinated  and record in the table as shown on the next page. (*Use forceps; be careful not to break roots! If part of a root breaks, ignore that root in the following step.)

2. Measure the length of root of all germinated seeds with unbroken roots. Record the data.

Measuring protocol: Before measuring, dry all the seeds by patting them with a paper towel to remove as much moisture as possible. For each sprout, measure the root length (not the shoot or seed itself) to the nearest mm. Measure when the root is straight. (If the root is curved, try to measure it as you roll it along a ruler.) Calculate the average length (mean) for each plate of seeds. Do not include seeds that did not germinate or seeds with broken roots when calculating average.​

Make a data table or list in your notebook

1. You will be assigned a salt lab day to mix up.​

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Multiple Choice

What type of dilution are we doing in this lab

• Serial

• Compound

• Mixed

Multiple Choice

How many seeds are we putting in each well of the well plate?

• 5-6

• 9

• 10

• 7-8

• 1

Multiple Choice

Does your data table / list have a spot for your assigned salt?

• Yes

• No

Multiple Choice

Does your data table / list have a spot for observations?

• Yes

• No

Multiple Choice

Does your data table / list have a spot for number of seeds in each well of the well plate?

• Yes

• No

Multiple Choice

Does your data table / list have a spot for length of the root for each seed?

• Yes

• No

​Example Data Table

​Well Plate & Concentration	​# of Seed	​# of Seed Germinated	​Length of Seed roots	​Average Length of germinated seeds
​A- 0.8M	​	​	​	​
B- 0.08M​	​	​	​	​
C- 0.008M​	​	​	​	​
D- 0.0008M​	​	​	​	​
E​- 0.00008M	​	​	​	​
F- 0 (Di Water)​	​	​	​	​

​Type of salt:_______________________