microscope

Microsope

Convert units

1 ​kilometre (km)	​1000 meters (m)
1 meter​ (m)	​100 centimetres (cm)
1 centimetre​ (cm)	10 millimetres​ (mm)
1 millimetre​ (mm)	1000 micrometres​ (μm
1 micrometre​ (μm	1000 nanometres​ (nm)

the smallest distance
that two objects can be apart and still appear as two separate objects (increase by sharpening with fine focus)

Resolution

how large an object
will appear compared to its actual size (increase by increasing objective lens)

Magnification

Key words

​Types of microscopes

There are two kinds of microscopes:

• Light

• Electron

Light microscopes use a beam of light to form an image of an object

Highest magnification x 2000

​It can distinguish two points 20 nm apart

Light microscope

There are two types of electron microscopes, transmission and scanning

They can magnify up to x 200000

They have a resolving power of up to 0.2nm

Electron microscopes

Microscope diagram

​	​
​Eyepiece lens	​this is the lens closest to your eye. It usually has a magnification of x 10
objective lens​	There are three lenses of different strengths which can be used to magnify the image more clearly​
​stage	This is the place where you put a microscope slide. clips hold the slide in place​
diaphragm​	​controls the amount of light that goes onto the microscope slide
light​	The light source projects light onto the microscope slide. It can be a mirror or an electric bulb ​
base​	this is very heavy to keep the microscope from falling over​
arm​	this holds the eyepiece lens above the stage​
fine focus control​	This is used to focus the image so it is very sharp and clear​
coarse focus control​	This is used to focus the image so that you can see it clearly​

​Drawing microscope slides / specimens

• use a pencil

• to draw only the lines you see. No shading or colouring

• Each circular diagram ( field of view ) should be about 3cm of the page in size

• If the cells you are viewing are similar it's useful to draw about 4-5 only

Multiple Choice

What is the smallest measurement

• 1nm

• 1mm

• 1 μm

Multiple Choice

what is the disadvantage of a light microscope

• low cost

• small magnification

• small size

Multiple Choice

which image has the worst resolution?

Multiple Choice

what is the largest measurement?

• 1mm

• 2000μm

• 5000 nm

What’s the largest measurement?

1 mm

2000 μm
5000
nm

1000 μm
2000 μm

5 μm

Multiple Choice

what is the advantage of an electron microscope?

• high cost

• high resolving power

• large size