BIOKEEN CHAPTER 7 (Set B)

The diagram below shows the steps in gene cloning by using plasmid.
Answer all questions based on this diagram.

30

METHODS IN GENE CLONING

A

B

C

D

E

Multiple Choice

1. 1. What is step B?

• Insertion

• Isolation of gene

• Screening

• Cleave/cut

Multiple Choice

2. 2. What is the name of the enzyme used in step B?

• Restricted enzyme

• Modifying enzyme

• Restriction enzyme

• Modified enzyme

Multiple Choice

4. 4. Identify step C.

• Cleave/cut

• Insertion

• Transformation & amplification

• Isolation

Multiple Choice

6. 6. The enzyme you had mentioned is used to seal the desired gene and vector together forming

• phosphodiester bond

• glycosidic bond

• peptide bond

• ester bond

Multiple Choice

8. 8. What is the importance of step D?

• To join both desired gene and vector

• To produce sticky or blunt ends

• To introduce the recombinant plasmid into host cells

• To undergo PCR process

Multiple Choice

10. 10. What is the X-gal used for in step E?

• To sweeten the agar

• To detect the activity of the plasmid

• To increase the strength of host cells

• To detect the activity of B-galactosidase

Multiple Choice

11. 11. What happen if the target genes were inserted into lacZ gene?

• lacZ gene activated

• lacZ gene producing B-galactosidase enzyme

• B-galactosidase enzyme activated

• lacZ gene disrupted and cannot encode B-galactosidase

Multiple Choice

13. 13. How to detect the colonies of bacteria are having the recombinant DNA?

• Colonies will be blue

• Colonies will be white

• Colonies will be grey

• Colonies will be pink