BIOKEEN CHAPTER 7 (Set B)

The diagram below shows the steps in gene cloning by using plasmid.
Answer all questions based on this diagram.

30

METHODS IN GENE CLONING

A

B

C

D

E

Multiple Choice

1. 1. What is step B?

• Insertion

• Isolation of gene

• Screening

• Cleave/cut

Multiple Choice

2. 2. What is the name of the enzyme used in step B?

• Restricted enzyme

• Modifying enzyme

• Restriction enzyme

• Modified enzyme

Fill in the Blanks

3. 3. Step B must use the same and compatible restriction enzyme because to produce

   sticky or blunt end.

Multiple Choice

4. 4. Identify step C.

• Cleave/cut

• Insertion

• Transformation & amplification

• Isolation

Fill in the Blanks

5. 5. What enzyme involved in step C?

Multiple Choice

6. 6. The enzyme you had mentioned is used to seal the desired gene and vector together forming

• phosphodiester bond

• glycosidic bond

• peptide bond

• ester bond

Fill in the Blanks

7. 7. Through step C,

   DNA/plasmid is produced.

Multiple Choice

8. 8. What is the importance of step D?

• To join both desired gene and vector

• To produce sticky or blunt ends

• To introduce the recombinant plasmid into host cells

• To undergo PCR process

Fill in the Blanks

9. 9. Suggest the method used in E.

Multiple Choice

10. 10. What is the X-gal used for in step E?

• To sweeten the agar

• To detect the activity of the plasmid

• To increase the strength of host cells

• To detect the activity of B-galactosidase

Multiple Choice

11. 11. What happen if the target genes were inserted into lacZ gene?

• lacZ gene activated

• lacZ gene producing B-galactosidase enzyme

• B-galactosidase enzyme activated

• lacZ gene disrupted and cannot encode B-galactosidase

Fill in the Blanks

12. 12. What is the function of B-galactosidase in the agar containing X-gal?

the X-gal.

Multiple Choice

13. 13. How to detect the colonies of bacteria are having the recombinant DNA?

• Colonies will be blue

• Colonies will be white

• Colonies will be grey

• Colonies will be pink