BIOKEEN CHAPTER 7 (Set A)

Multiple Choice

1. 1. What is DNA recombinant technology?

• Techniques for sequencing and manipulating gene

• Techniques in carrying fragment DNA into plasmid

• Joining together DNA molecules of 2 different sources to produce new genetic combinations

• Joining a plasmid to the host cell to produce new organism

Multiple Choice

2. 2. These are the tools used in recombinant DNA technology EXCEPT

• Modifying enzymes

• Target RNA

• Host cell

• Restriction enzymes

RECOMBINANT DNA TECHNOLOGY

Answer questions no. 3 - 9 based on this diagram.

Learning outcomes7.1 Recombinant DNA Technology
b)

Lists tools used in recombinant DNA technology and state their function

10

Multiple Choice

3. 3. What is target DNA?

• Gene to be copied

• Gene to be cloned

• Gene to be destroyed

• Gene to be inserted into host cell

Multiple Choice

7. 7. Examples of DNA cloning vectors as below EXCEPT

• Bacteriophage

• Cosmid

• YAC

• Bacteria

Multiple Choice

8. 8. Host cell must be

• able to replicate independently in the vector

• able to accept foreign DNA in MCS

• able to accept recombinant DNA by transformation

• Has selectable marker gene

Multiple Choice

10. 10. What is the purpose of using PCR?

• To amplify a small amount of DNA

• To duplicate the DNA

• To cut/cleave the DNA

• To denature the double stranded of DNA

Multiple Choice

11. 11. Choose the correct step in PCR.

• Denaturation : temperature 50⁰C

• Elongation : temperature 72⁰C

• Annealing : temperature 90⁰C