DNA TRANSCRIPTION

DNA Transcription

​What do I know about DNA Transcription?

Open Ended

What I KNOW about DNA Transcription is that...

1. Describe the process of DNA transcription; and

2. Outline the events that occur during DNA transcription.

​I can:

Open Ended

What I KNOW about DNA Transcription is that...

​What is Transcription?

​ TRANSCRIPTION is converting a DNA sequence to RNA

​What is the purpose of Transcription?

​Since the DNA cannot leave the nucleus, the sequence is rewritten into messenger RNA through the process of TRANSCRIPTION so the information can be brought to the ribosomes for translation into proteins.

What are the events that take place during DNA TRANSCRIPTION?

TRANSCRIPTION Events

• Initiation

• Elongation

• Termination

INITIATION

• Transcription factor recognise the promoter region or TATA box & bind to the promoter.

• RNA polymerase binds to transcription factor (at promoter) to create Transcription Initiation Complex.

• RNA polymerase then starts transcription.

ELONGATION

• RNA polymerase adds RNA nucleotides to the 3^' end of the growing RNA molecule as it continues along the double helix.

• The new RNA molecule peels away from its DNA template & the DNA double helix reforms.

TERMINATION

• The RNA polymerase continue to transcribing for hundreds of nucleotide past the terminator sequence, AAUAAA.

• At a point about 10 to 35 nucleotide past this sequence ( AAUAAA), the pre-mRNA is cut from the RNA polymerase.

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Multiple Choice

Where does Transcription take place?

• Inside the nucleus

• Inside the cytoplasm

• Inside the Ribosome

• Inside the Rough ER

Multiple Choice

What happens to the mRNA sequence after it has been synthesized?

• stays in the nucleus

• leaves the cell

• leaves the nucleus

• separates into smaller pieces

Multiple Choice

Process when the code from the DNA is copied to mRNA

• Transcription

• Translation

Multiple Choice

Which of the following steps is NOT part of TRANSCRIPTION in eukaryotes?

• Initiation

• Elongation

• Termination

• Transformation

Multiple Choice

What enzyme does transcription use?

• DNA polymerase

• ligase

• RNA polymerase

• helicase

Multiple Choice

Transcribe the following DNA sequence to the complementary mRNA sequence.

TAC CGG CTG

• AUG GCC GAC

• AUG GCC GAG

• AUG CGG GAC

• GUA GCC GAC