Post Lab Experiemnt 1

POST LAB EXP 1
BASIC TECHNIQUE IN MICROSCOPY

Open Ended

List down the learning objective from last week experiment.

Poll

Rate the difficulty of last week experiment.

• Easy Peasy

• So-so

• A bit Hard

• Super challenging

Word Cloud

What did you find difficult about last week experiment?

Lets look at the lab report

​Copy from the LAB MANUAL

​

Title &
Objectives

Introduction questions

Based in the theory found in the lab manual

Fill in the Blanks

What is a light compound microscope?

an instrument that can be use to focus and magnify

specimens.

Multiple Choice

Give the formula to calculate the actual magnification

• Actual magnification of a specimen is magnification power of objective lens X magnification power of objective lens.

• Actual magnification of a specimen is magnification power of objective lens X magnification power of ocular lens.

• Actual magnification of a specimen is magnification power of ocular lens X magnification power of ocular lens

Fill in the Blanks

What is the field of view?

Simplest method of estimating linear dimension is to compare the size of the image to the

of the field of view

Multiple Select

Which of the following is the correct writing of procedure format. (Can choose more than one)

Procedure (3 marks)

Multiple Choice

Which of the following is the correct format for drawing. (Can choose more the one)

Results: Drawing (5 marks)

Title - 1m
Drawing -3m
Actual magnification - 1m

​2m





2m





1m

Results: Table
(5 marks)

Multiple Select

1.           Determine the position of ‘e’. Give 2 reasons. (Can choose more than one)

• Image under microscope is inverted and larger

• Inverted because the lens is concave

• Inverted because the lens is convex

• Larger because the biconvex shape magnify the image

Dropdown

2.           Determine what happens to the depth of field when the power of an objective lens increases. Explain why in terms of focal length.

The depth of field ​

Select answer

.

The wider the lens, the ​

Select answer

the focal length.

Fill in the Blanks

3.           Compare location of threads observed under naked eye and microscope

Location of threads observed under naked eye and microscope is

Fill in the Blanks

4.           State what happens to image size when observed under microscope.

Cells are

and clearer

Dropdown

State the significance why some cells need to be stained.

    Some cells are stained to show ​
Select answer
with the background

Open Ended

Write down atleast 3 conclusion from the experiment.

QUESTIONS

Part A 

For Questions: 1 to 7, choose the correct answer from the following list: 

 A Scanning objective lens (4x) B Low-power objective lens (10x) C High-power objective lens (40x) D Oil immersion objective lens (100x) 

Drag and Drop

Which is the shortest objective lens?  ​​

Placeholder

Drag these tiles and drop them in the correct blank above

B

C

D

A

Drag and Drop

Which objective lens should you use when you begin to focus a specimen?   ​
Placeholder

Drag these tiles and drop them in the correct blank above

A

B

C

D

Drag and Drop

Which objective lens should be in position before you store a microscope?    ​
Placeholder

Drag these tiles and drop them in the correct blank above

A

B

C

D

Drag and Drop

Which objective lens will deliver the highest amount of light?     ​
Placeholder

Drag these tiles and drop them in the correct blank above

A

B

C

D

Drag and Drop

Which objective lens requires immersion oil to fill up the space between the specimen and the lens?     ​ ​
Placeholder

Drag these tiles and drop them in the correct blank above

A

B

C

D

Drag and Drop

Which objective lens will still remain in focus when placed at the longest working distance from the specimen?     ​ ​
Placeholder

Drag these tiles and drop them in the correct blank above

A

B

C

D

Drag and Drop

When using an ocular lens with 10x magnification power, which objective lens should be used to obtain the following actual magnification?

a. 100 times of its diameter ……​
Placeholder
………….

b. 1000 times of its diameter ……​
Placeholder
………….

Drag these tiles and drop them in the correct blank above

A

B

C

D

QUESTIONS

Part B 

1. Using a 40x objective lens, determine the length and width of a cell from a piece of cork tissue with approximately 20 cells in horizontal position and 10 cells in vertical position.    

Fill in the Blanks

The size of a cork cell :

Under 40X objective lens = 500 μm

Width for 20 cells  = 500 μm

                   1 cell    = 500 / 20 = 25 μm

Length for 10 cells = 500 μm

                    1 cell = 500 / 10 = 50 μm

The size of a cork cell =

μm²

Fill in the Blanks

2. Based on laboratory practices, what do you use to clean the microscope lenses?  

tissue

Fill in the Blanks

3. While observing a moving microorganism under a microscope, you found that the organism has moved out of the field of view to the right. In order to keep observing the microorganism, which direction do you move your slide (right/left)?

Dropdown

4. How do you adjust the slide when the specimen is out of the field of view to the top? 

​

Select answer

you / me

QUESTIONS

 Part C 

Complete the following sentences. 

Fill in the Blanks

a. A microscope is called a compound microscope when it consists of more than one set of

Fill in the Blanks

 b. Condenser and iris diaphragm are useful to coordinate

End of
Post Lab 1