Micropipettes Lesson

Micropipettes

Used to measure small quantities of liquids

Unit is measure is the microliter (μL), 1,000μL = 1mL

1mL of water weighs 1g

Handle carefully, be gentle
Change tips between solutions; minimize number of changes
Never touch tip to anything
Don't lay it down with liquid in the tip

Micropipettes

To draw up liquid:
1. Depress plunger to first stop.
2. Place tip vertically into the liquid. Avoid touching sides or bottom.
3. Gently release plunger.
4. Remove tip from liquid.

To expel liquid:
5. Place tip into receiving vessel.
6. Press plunger to second stop.
7. Pull tip out of liquid before releasing.

Micropipettes

Are rated by volume range, named by max volume
e.g. P20, P100, P200, P1,000

Read place values by placing the max volume in the window, starting at the top

Choose the smallest pipette you can for the task at hand

Never turn the dial to a value outside of that range

Multiple Choice

When using a micropipette you should go to which stop prior to taking in liquid?

• 1st

• 2nd

• none, enter the liquid first

• either, it doesn't effect your results

Multiple Choice

When trying to keep the samples sterile, when is it appropriate to change the tip of the micropipette?

• If you change samples

• If it touches you

• If it touches the table

• All of these are correct

Multiple Choice

What tool would you use to measure 325 μl?

• P-20

• P-200

• P-1000

Fill in the Blanks

How many microliters are in 1.5 milliliters (just add number, not units)

Fill in the Blanks

How many microliters are in 0.85 milliliters (just add number, not units)

Multiple Choice

If a laboratory protocol asks you to add 0.65 milliliters of reagent solution to a tube, which micropipette should you use?

• p20 pipette

• p200 pipette

• p1000 pipette

Fill in the Blanks

How many microliters is this p1000 pipette set to dispense?

Fill in the Blanks

How many microliters is this p200 pipette set to dispense? (enter just the number)

Fill in the Blanks

Indicate the quantity represented on this P50.

Fill in the Blanks

State the quantity indicated on this P200.